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KMID : 1123920050190041062
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 4 p.1062 ~ p.1067
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Inhibition Effect of Zizania latifolia on Apoptosis Induced by H2O2 in Neuro2A Cell
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Park Won-Hyung
Cha Yun-Yeop
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Abstract
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The purpose of this study was to examine the inhibition effect of Zizania latifolia that has been used heart disease, Diabetes Mellitus and Skin disease for a long time on apoptosis induced by in Neuro2A cell. Neuro2A cells were cultivated in RPMI(GibcoBRL) with , FBS and treated with , and Zizania latifolia. We measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined by using western blot. The cell viability in Zizania latifolia treatment (60ug/ml<) decreased significantly compared with that of none treatment. (p<0.001) Zizania latifolia increased cell viability about twice as much as that being injury by . (Zizania Latifolia 20ug/ml, 200uM, P<0.001) DNA fragmentation developed by , but was not developed in Zizania latifolia treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by but were not activated in Zizania latifolia treatment.. P53, P2l and Bu activated by , and Bcl-2 got into inactivation. But the opposite results appeared in Zizania latifolia treatment. In conclusion, these results suggest that Zizania latifolia inhibit the development of DNA fragmentation and apoptosis by and the antioxidant action of Zizania latifolia is effective. More researches about effect of Zizania latifolia are considered to need.
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KEYWORD
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Zizania latifolia, Neuro2A cell, Apoptosis
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